The Elements of Bacteriological Technique - LightNovelsOnl.com
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10. _Saturated Aqueous Solution._
Weigh out
Basic fuchsin 1.5 grammes
and proceed as in preparing the corresponding solution of methylene-blue (_q. v._).
11. _Saturated Alcoholic Solution._
Weigh out
Basic fuchsin 3.5 grammes
and proceed as in preparing the corresponding solution of methylene-blue.
12. _Carbolic Fuchsin_ (Ziehl).
Weigh out
Basic fuchsin 1.0 gramme Carbolic acid 5.0 grammes
dissolve in
Distilled water 100.0 c.c.
and add
Absolute alcohol 10.0 c.c.
Filter.
CONTRAST STAINS.
~Eosin.~--There are several commercial varieties of eosin, which, from the bacteriological point of view, possess very different values. Gruebler lists four varieties, of which two only are useful for bacteriological work:
Eosin, aqueous yellowish.
Eosin, aqueous bluish.
13. _Eosin Aqueous Solution_ (Yellowish or Bluish Shade), 1 per cent.
Weigh out
Eosin, aqueous 1.0 gramme
dissolve in
Distilled water 100.0 c.c.
and add
Absolute alcohol 5.0 c.c.
Filter.
14. _Eosin Alcoholic Solution_, 0.5 per cent.
Weigh out
Eosin, alcoholic 0.5 gramme
and dissolve in
Alcohol (70 per cent.) 100.0 c.c.
Filter.
~Safranine.~--
15. _Aqueous Solution._
Weigh out.
Safranine 0.5 gramme
and dissolve in
Distilled water 100.0 c.c.
Filter.
~Neutral Red.~--
16. _Aqueous Solution._
Weigh out
Neutral red 1.0 gramme
and dissolve in
Distilled water 100.0 c.c.
Filter.
~Vesuvin (or Bismarck Brown).~--
17. _Saturated Aqueous Solution._
